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- Send leaf samples to Raleigh for virus testing. Screen for 9 viruses and 1 phytoplasma and 1 bacteria.
- Sterilize paper plates or paper towels (fast exhaust, 15 minutes) in autoclave bag with tape- or use sterile Petri dishes
- Sterilize small flasks for sterilizing plant material
- Sterilize flask of water (slow exhaust, 20 minutes) with foil cover (use for rinsing plant material after surface sterilized in 15% bleach
- Check distilled water level (do I need to make more)
- Check HCL and NaOH level (do I need to make more)
- Make media (1L makes about 13 magenta boxes (70mL’s each)
- Pour media into magenta boxes
- Autoclave (slow exhaust, 20 minutes)
- Place trays of magenatas in the laminar flow hood to cool overnight
