TC Media Recipes

Current NCSU PTL Recipe (1 L)

1. Add 500 mL of diH2O to a >1000 mL beaker

2. Insert magnetic stir rod and place on stir plate at 125 speed

3. Add each item below individually and allow to dissolve before adding next ingredient:

2.3 g McCown Woody Plant Basal Salt Mixture

6 mL Thiamine (stock solution = 10 mg/ 100 mL)

1 mL 1000x MS vitamins

20 g Sucrose

0.1 g Myo-inositol

2 mL Zeatin (1 mg/ mL)

0.2 g Sequestrene

1.5 g MES

4. Adjust volume to 1 L

5. Calibrate pH meter

6. Bring pH up to 5

7. Add 4 g Agar

8. Autoclave on Liquid 20 setting (Should take 30-45 minutes)

9. Remove from autoclave and allow to cool for ~10 minutes

10. Pour media into Magenta boxes or culture tubes and allow to set and cool for ~1 hour or over night

Older Recipes:

MSU Recipe (1 L)

1. 2.3 g Woody Plant Media (phytotech)
2. 1 ml MS vitamins (sigma M3900)
3. 556 mg Ca(NO)3-4H2O (Sigma)
4. 20 g sucrose (phytotech)
5. 2 mg zeatin riboside (phytotech)
6. pH 5
7. 4 g agar (phytotech)

UGA Recipe (1L)

1. 2.3 g WPM
2. 1 ml MS vitamins
3. 556 mg Ca(NO)3-4H2O
4. 20 g sucrose
5. 2 mg zeatin riboside
6. pH 5
7. 3.5 g agar

NCSU Recipe (1L)

1. 2.3 g WPM
2. 6 mL Thiamine
3. 5 mL MS vitamins
4. 30 g sucrose
5. .1 g myo-Inositol
6. 2 mg zeatin
7. pH 5.2
8. 6 g agar